rc2 (Developmental Studies Hybridoma Bank)

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Developmental Studies Hybridoma Bank rc2

(A,B) Increased pS6 expression in TSC1/2 cKO cortex. (C) Quantification of changes in pS6 expression in TSC1/2 cKO cortex (n = 6 mice/group). (D) Immunoblot analysis and quantification (E) of increased pS6 expression in TSC1/2 cKO cortex (n = 4 mice/group). (F,G) Increased pS6 expression in TSC1/2 cKO RGCs (arrowhead). (H) Quantification of changes in pS6 expression in TSC1/2 cKO RGC soma (n = 25 soma). RGCs were co-labeled with <t>RC2</t> antibodies. CP, cortical plate; IZ, intermediate zone; VZ, ventricular zone. Data shown are mean ± SEM. Unpaired two-sided t -test; ***p < 0.001 [ p = <0.0001 (C), 0.0001 (E) , <0.0001 (H)]. Scale bar: A-B (100 μm); F-G (50 μm). Sample RGC soma are outlined (dotted lines; F-G). A.U., arbitrary unit of pS6 intensity normalized to background. For immunoblot source data, see Supplementary Fig. 1 .

Rc2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rc2/anti-Nestin%2Fradial+glial+marker/pmc13052830-364-97-107
Average 94 stars, based on 15 article reviews

rc2 - by Bioz Stars, 2026-10

94/100 stars

Images

1) Product Images from "TSC tunes progenitor balance and upper-layer neuron generation in neocortex"

Article Title: TSC tunes progenitor balance and upper-layer neuron generation in neocortex

Journal: Nature

doi: 10.1038/s41586-025-09810-5

Figure Legend Snippet: (A,B) Increased pS6 expression in TSC1/2 cKO cortex. (C) Quantification of changes in pS6 expression in TSC1/2 cKO cortex (n = 6 mice/group). (D) Immunoblot analysis and quantification (E) of increased pS6 expression in TSC1/2 cKO cortex (n = 4 mice/group). (F,G) Increased pS6 expression in TSC1/2 cKO RGCs (arrowhead). (H) Quantification of changes in pS6 expression in TSC1/2 cKO RGC soma (n = 25 soma). RGCs were co-labeled with RC2 antibodies. CP, cortical plate; IZ, intermediate zone; VZ, ventricular zone. Data shown are mean ± SEM. Unpaired two-sided t -test; ***p < 0.001 [ p = <0.0001 (C), 0.0001 (E) , <0.0001 (H)]. Scale bar: A-B (100 μm); F-G (50 μm). Sample RGC soma are outlined (dotted lines; F-G). A.U., arbitrary unit of pS6 intensity normalized to background. For immunoblot source data, see Supplementary Fig. 1 .

Techniques Used: Expressing, Western Blot, Labeling

other:

Article Title: The REST remodeling complex protects genomic integrity during embryonic neurogenesis
Article Snippet: Mouse anti-a-tubulin (clone AA4.3, 1:5000), mouse anti-actin (clone JLA20, 1:1000), anti-RC2 (1:100) and mouse anti-BrdU (clone G3G4, 1:200), were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biology.

Article Title: Evidence That Descending Cortical Axons Are Essential for Thalamocortical Axons to Cross the Pallial-Subpallial Boundary in the Embryonic Forebrain
Article Snippet: The following primary antibodies were used: anti-Apc (ab15270), rabbit, 1∶100 (Abcam); anti-Pax6 (PAX6), mouse, 1∶200 (Developmental Studies Hybridoma Bank [DSHB]); anti-L1 (MAB5272), rat, 1∶500 (Chemicon International); anti-Tag1 (4D7/TAG1), mouse, 1∶100 (DSHB); anti-Gsh2, mouse, 1∶1500 ; anti-microtubule associated protein (anti-MAP2) (AB5622), mouse, 1∶500 (Upstate Cell Signalling Solutions); anti-neurofilament (NA1297), mouse, 1∶500 (Biomol International, L.P.); anti-RC2 (RC2), mouse, 1∶100 (DSHB).

Article Title: A “Hit and Run” Approach to Inducible Direct Reprogramming of Astrocytes to Neural Stem Cells
Article Snippet: Samples were mounted in anti-fade DAPI mounting media (Invitrogen) and photographed on a Leica SP5 confocal microscope.

Article Title: The REST remodeling complex protects genomic integrity during embryonic neurogenesis
Article Snippet: Mouse anti-α-tubulin (clone AA4.3, 1:5000), mouse anti-actin (clone JLA20, 1:1000), anti-RC2 (1:100) and mouse anti-BrdU (clone G3G4, 1:200), were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biology.

Article Title: Alix is required during development for normal growth of the mouse brain
Article Snippet: Anti-RC2 , Mouse , DSHB (AB_531887) , 10 , , .

Western Blot:

Article Title: FIP200 is required for maintenance and differentiation of postnatal neural stem cells
Article Snippet: .. Primary antibodies used were mouse anti-Nestin (1:50, Rat-401), anti-PSA-NCAM (1:50, 5A5), anti-BrdU (1:200, G3G4), anti-RC2 (1:100, C-19)(all from DSHB, IA), Rhodamine conjugated anti-BrdU (1:200, Millipore, MA), anti-beta-actin (1:5000), anti-vinculin (1:5000; both from Sigma, MO) and anti-NeuN (1:500, Millipore, MA); rabbit anti-p53 (1:1000, Leica, Germany), anti-Ki67 (1:100, Spring Bioscience, CA), anti-p62 (1:200 for immunofluorescent and 1:1000 for Western blot, Enzo, PA), anti-GFAP (1:400, DAKO, CA), anti-LC3 (1:1000, Cell Signaling, MA), anti-PAX6 (1:100, Abcam, Cambridge, UK), anti-Tuj1 (1:100, Sigma, MO), anti-SOX2 (1:500, Millipore, MA) and anti-phosphor-STAT3 (Tyr 705) (1:100, Cell Signaling, MA); rat anti-Ki67 (1:100, Biolegend, CA). ..

Incubation:

Article Title: In utero gene therapy rescues microcephaly caused by Pqbp1 -hypofunction in neural stem progenitor cells
Article Snippet: .. Sections were subject to dual immunostains by overnight incubation at 4 °C with anti-PQBP1 goat antibody (1:500, C-20, Santa Cruz Biotechnology), anti-PQBP1 rabbit antibody (1:400, FL-265, Santa Cruz Biotechnology), anti-RC2 I mouse IgM antibody (1:200, Developmental Studies Hybridoma Bank, DSHB), anti-Sox2 rabbit antibody (1:200, Santa Cruz Biotechnology), anti-Tuj-1 mouse antibody (1:500, Sigma), mouse anti-MAP2 antibody (1:500, Sigma), anti-PAX6 mouse antibody (1:100, Developmental Studies Hybridoma Bank, DSHB), anti-Tbr2 rabbit antibody (1:500, Millipore), rabbit anti-Foxp1 antibody (1:200, abcam), anti-Cux1 rabbit antibody (1:200, abcam) or anti-GAD67 mouse antibody (1:50, Millipore). .. Secondary staining was with biotin anti-goat IgG (1:500, Molecular Probes) combined with tyramide signal amplification (TSA Biotin System, PerkinElmer Life and Analytical Sciences) and Alexafluor-488 or Cy3 labeled secondary antibodies (1:500, Invitrogen).

Immunostaining:

Article Title: Brain heterotopia formation by ciliopathic breakdown of neuroepithelial and blood‐cerebrospinal fluid barriers
Article Snippet: .. The primary antibodies used for immunostaining included chicken anti‐GFP (Aves Labs, USA, 1:1000), rabbit anti‐GFP (1:1000, Invitrogen, USA), rat anti‐Pdgfrα (1:300, Millipore) rabbit anti‐ACIII (1:1000, Santa Cruz Biotechnology, USA), rabbit anti‐Blbp (Chemicon, USA, 1:500; Abcam, 1:300), mouse anti‐RC2 (DSHB, USA, 1:200), rat anti‐Ctip2 (Abcam, 1:1000), rabbit anti‐Ki67 (Lab Vision, USA, 1:200), rabbit anti‐Wnt3a (Cell Signaling Technology, USA, 1:1000), rabbit anti‐Pdgf‐A (Abbiotec, USA, 1:1000), rabbit anti‐Tbr2 (Abcam, USA, 1:300), rabbit anti‐Claudin‐5 (United States Biological, USA, 1:300), rabbit anti‐Par3 (Millipore, USA, 1:300), rabbit anti‐Notch1 (Cell Signaling Technology, USA, 1:400), Alexa Fluor 546‐conjugated streptavidin, TRITC‐conjugated phalloidin (Invitrogen, USA, 1:1000), mouse anti‐aTub (Sigma, USA, 1:1000), rabbit anti‐β‐Catenin (Cell Signaling Technology, USA, 1:500), rabbit anti‐Pax6 (Covance, USA, 1:300), rabbit phospho‐Histone H3 (Santa Cruz Biotechnology, USA, 1:1000), rabbit anti‐Pdgfrβ (Cell Signaling Technology, USA, 1:300), biotin‐conjugated 70 kDa dextran (Invitrogen, USA), biotin‐conjugated cadaverine (Invitrogen, USA), mouse anti‐Desmin (DAKO, Denmark, 1:1000), rat anti‐ICAM1 (Abcam, USA, 1:500) and rabbit anti‐Olig2 (Millipore, USA, 1:300). .. Images were acquired using an upright Nikon C1 spectral confocal microscope equipped with 408, 488, 561, and 640 nm lasers with 10×, 20×, and 63× objectives.

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